tibetica Cui 9459 JF706327 JF706333 Cui and Zhao 2012 P. tibetica Cui 9457 JF706326 JF706332 Cui and Zhao 2012 P. truncatospora Cui 6987 JN048778 HQ654112 Cui et al. 2011 P. truncatospora Dai 5125 HQ654098 HQ848481 Zhao and Cui 2012 P. vicina MUCL 44779 FJ411095 FJ393862 Robledo et al. 2009 Pe. chaquenia MUCL 47647 FJ411083 FJ393855 Robledo
et al. 2009 Pe. chaquenia MUCL 47648 FJ411084 FJ393856 Robledo et al. 2009 Pe. micropora MUCL43581 FJ411086 FJ393858 Robledo et al. 2009 Pe. neofulva MUCL 45091 FJ411080 FJ393852 Robledo et al. 2009 Pe. pendula MUCL 46034 FJ411082 FJ393853 Robledo et al. 2009 Pyrofomes demidoffii MUCL 41034 FJ411105 FJ393873 Robledo et al. 2009 aselleck products sequences newly generated in this study Sequences were aligned with additional sequences downloaded from GenBank (Table 1) using BioEdit (Hall 1999) and ClustalX (Thomson et al. 1997). Alignment selleck chemical was manually adjusted to allow maximum alignment and to minimize gaps. Sequence alignment was deposited selleck chemicals at TreeBase (http://purl.org/phylo/treebase/; submission ID 12083). Maximum parsimony analysis was applied to the combined ITS and nLSU datasets. In phylogenetic reconstruction, sequences of Donkioporia expansa (Desm.) Kotl. & Pouzar and Pyrofomes demidoffii (Lév.) Kotl. & Pouzar obtained from GenBank were used as outgroup. The tree construction procedure was performed in PAUP* version 4.0b10 (Swofford 2002) as described by Jiang et al. (2011). All characters were equally weighted
and gaps were treated as missing data. Trees were inferred using the heuristic search option with TBR branch swapping and 1,000 random sequence additions. Max-trees
were set to 5,000, branches of zero length were collapsed and all parsimonious BCKDHB trees were saved. Clade robustness was assessed using a bootstrap (BT) analysis with 1,000 replicates (Felsenstein 1985). Descriptive tree statistics tree length (TL), consistency index (CI), retention index (RI), rescaled consistency index (RC), and homoplasy index (HI) were calculated for each Maximum Parsimonious Tree (MPT) generated. MrMODELTEST2.3 (Posada and Crandall 1998; Nylander 2004) was used to determine the best-evolution for each data set for Bayesian inference (BY). Bayesian inference was calculated with MrBayes3.1.2 with a general time reversible (GTR) model of DNA substitution and a gamma distribution rate variation across sites (Ronquist and Huelsenbeck 2003). Four Markov chains were run for 2 runs from random starting trees for 2 million generations, and trees were sampled every 100 generations. The first one-fourth generations were discarded as burn-in. A majority rule consensus tree of all remaining trees was calculated. Branches that received bootstrap support for maximum parsimony (MP) and Bayesian posterior probabilities (BPP) greater or equal than 75 % (MP) and 0.95 (BPP) respectively were considered as significantly supported. Results Taxonomy Perenniporia aridula B.K. Cui & C.L. Zhao, sp. nov. (Figs. 1 and 2) Fig.