Other genes which are www.selleckchem.com/products/necrostatin-1.html differentially expressed are closely to carcinogenesis such as cell cycle, cell invasion and apoptosis. In table 1, the most changed genes comparing FA3 group and DMH group are listed, among which are some oncogenes, for example, www.selleckchem.com/products/VX-680(MK-0457).html Oil (oncoprotein induced transcript 1), Tnfrsf11b (tumor necrosis factor receptor superfamily, member 11b), Hmgn5 (high-mobility group nucleosome binding
domain 5) are down-regulated while tumor suppressors such as Hnf4a (hepatic nuclear factor 4, alpha), Cdhr2 (cadherin-related family member 2), Muc2 (mucin 2) are up-regulated. From the results of the microarray analysis, we selected 5 genes i.e., K-ras, c-MYC, DNMT1, Tpd52, CDKN1b for PCR confirmation because they are already considered as tumor-related genes. The primers for these genes are shown in Table 2. Table 1 List of the most differentially expressed genes whose changes due to DMH treatment could be reversed by folic acid Accession number Gene symbol Gene Description Fold change P value Downregulated genes NM_207634 Rps24 ribosomal protein S24 (Rps24), transcript variant 2 0.002356454 2.05154E-06 NM_012052 Rps3 ribosomal protein S3 (Rps3) 0.00933479 6.38113E-06 NM_033073
Krt7 keratin 7 0.024674534 0.001286211 NM_024478 Grpel1 GrpE-like 1, mitochondrial (Grpel1) 0.029123617 3.65271E-05 NM_024243 Fuca1 fucosidase, alpha-L- 1 0.031740456 0.000162318 NM_146050 Oit1 oncoprotein induced transcript 1 0.032247549 0.001799574 NM_013614 Odc1 ornithine decarboxylase, structural Florfenicol MRT67307 price 1 0.032361 4.48641E-05 NM_025431 Llph LLP homolog, long-term synaptic facilitation (Aplysia) 0.036784284 1.18163E-06 NM_008764 Tnfrsf11b tumor necrosis factor receptor superfamily, member 11b 0.041187965 7.03729E-05 NM_009402 Pglyrp1 peptidoglycan recognition protein 1 0.041272749 0.009299333 NM_010106 Eef1a1 eukaryotic translation elongation factor 1 alpha 1 0.041438052 7.22246E-06 NM_001008700
Il4ra interleukin 4 receptor, alpha 0.043141894 0.000223171 NM_182930 Plekha6 pleckstrin homology domain containing, family A member 6 0.04544609 0.001545018 NM_011463 Spink4 serine peptidase inhibitor, Kazal type 4 0.045587012 0.000688366 NM_016710 Hmgn5 high-mobility group nucleosome binding domain 5 0.046928235 0.000333311 NM_016981 Slc9a1 solute carrier family 9 (sodium/hydrogen exchanger), member 1 0.052191789 5.29847E-05 NM_145533 Smox spermine oxidase (Smox), transcript variant 2 0.053274908 6.23127E-05 NM_008305 Hspg2 perlecan (heparan sulfate proteoglycan 2) 0.056450624 0.001205571 NM_172051 Tmcc3 transmembrane and coiled coil domains 3 0.058793481 0.001122075 NM_009768 Bsg basigin (Bsg), transcript variant 1 0.061259044 0.000407939 Upregulted genes NM_009946 Cplx2 complexin 2 1109.786672 0.000155322 NM_001039493 Plekhm3 pleckstrin homology domain containing, family M, member 3 56.2494337 0.000450001 NM_024272 Ssbp2 single-stranded DNA binding protein 2 (Ssbp2), transcript variant 2 54.215495 2.06403E-05 NM_175013 Pgm5 phosphoglucomutase 5 47.