“Though the role of reactive oxygen species (ROS) in female infertility has been a subject of rigorous research worldwide, there is inadequate information on the cut-off value of ROS in the oocyte microenvironment beyond which ART outcome may be adversely affected. An upper ROS level in follicular fluid (FF) samples of women undergoing IVF beyond which good quality embryo formation is unlikely, is established. ROS, lipid peroxidation and total antioxidant capacity were estimated. The upper cut-off ROS level beyond which viable embryo formation is not favorable was found to be similar to 107
cps/400 mu l FF. This level, determined in women with tubal factor infertility, was further validated click here in women with endometriosis and PCOS and correlated with fertilization and pregnancy rate and embryo quality. Summarizing, a threshold level in FF has been established for the first time beyond which ROS may be considered toxic for viable embryo formation and pregnancy outcome. (C) 2010 Elsevier Inc. All rights reserved.”
“Objective: To assess the feasibility of sealing the thoracic duct (TD) in dogs using ultrasonically selleck chemical activated shears via thoracoscopy.\n\nStudy Design: In vivo experimental study.\n\nAnimals:
Mature dogs (n = 6).\n\nMethods: Dogs were anesthetized without pulmonary exclusion and positioned in left lateral recumbency. Lymphangiography was performed to identify TD anatomy. Methylene blue was injected into the lymphatic catheter to identify the TD and its branches. Under thoracoscopic guidance (right dorsal 8-10th intercostal spaces), the TD was sealed with an ultrasonic device and lymphangiography was repeated. If the flow of contrast continued beyond the occlusion site, additional attempts to seal the duct were CB-839 made. Dogs were euthanatized, the TD was excised and fixed in formalin for histopathology.\n\nResults: Thoracoscopic
identification of the TD was possible in 5 dogs. Three dogs required conversion to a thoracoscopic-assisted approach and 3 dogs required resealing of the TD closer to the diaphragm. Thoracic duct occlusion (TDO) was ultimately achieved in all 6 dogs based on follow-up lymphangiography. TDO by tissue coagulation was confirmed by histopathology.\n\nConclusions: Thoracoscopic identification and occlusion of the TD using ultrasonically activated shears with bilateral lung ventilation is technically feasible in normal dogs and provides a less invasive alternative to open thoracotomy procedures.”
“To investigate the susceptibility of Sri Lankan new entry university students to varicella zoster virus (VZV) infection, a cross-sectional descriptive study was carried out among new entrant medical and engineering students of the University of Peradeniya, Sri Lanka. Self-reported history of chicken pox was studied first, followed by serological evaluation for VZV IgG antibodies.