Endogenous cellular Psor co-precipitated with endogenous beta 6 a

Endogenous cellular Psor co-precipitated with endogenous beta 6 and colocalised with alpha v beta 6 at the cell membrane and intracellular vesicles. Knockdown of Psor, with small interfering RNA, had no effect on alpha v beta 6-dependent adhesion or migration but abrogated alpha v beta 6-mediated oral carcinoma cell invasion both in Transwell and, the more physiologically relevant, organotypic invasion assays, recapitulating

the behaviour of the beta 6-mutant cell line. Membrane-permeant Tat-peptides encoding the unique C-terminal residues of beta 6, bound directly to DUB inhibitor recombinant Psor and inhibited cellular Psor binding to beta 6; this blocked alpha v beta 6-dependent, but not alpha v beta 6-independent, invasion. These data identify a novel interaction between Psor and beta 6 and demonstrate that it is required for alpha v beta 6-dependent invasion by carcinoma cells. Inhibition of this interaction may represent a novel therapeutic

strategy to target carcinoma invasion. Oncogene (2011) 30, 1422-1435; doi:10.1038/onc.2010.535; published online 6 December 2010″
“Background Poppers are a recreational substance of abuse belonging to the alkyl nitrite family of compounds. In the United Kingdom, where they are legal to purchase but illegal to sell for human consumption, 10% of the general population have tried them. They are considered low risk to physical and mental health. Two recent case series from France demonstrated foveal Cl-amidine inhibitor pathology in individuals associated with poppers use.\n\nMethod

A case series of seven patients presenting to four hospitals in the United Kingdom with visual impairment and maculopathy associated with inhalation of poppers.\n\nResults All patients experienced visual symptoms associated with poppers use. The majority had impaired visual acuity, central scotomata, distortion, or phosphenes. Clinical signs on fundoscopy ranged from normal foveal appearance to yellow, dome-shaped lesions at the foveola. Spectral domain optical coherence tomography (SD-OCT) showed varying degrees of disruption of the presumed inner segment/outer see more segment (IS/OS) junction.\n\nDiscussion Although poppers have been in use for several decades, in 2007, following legislative changes, there was a change in the most commonly used compound from isobutyl nitrite to isopropyl nitrite. There were no reports of ‘poppers maculopathy’ before this. Poppers maculopathy may be missed if patients are not directly questioned about their use. The disruption or loss of the presumed IS/OS junction on SD-OCT are a characteristic feature. Further study of maculopathy in poppers users is now needed. Raising public awareness of the ocular risks associated with their use may be necessary. Eye (2012) 26, 1479-1486; doi:10.1038/eye.2012.

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