The relative standard deviation (RSD) and assay values obtained by two analysts were 0.28, 99.67, and 0.26, 99.68, respectively selleck screening library [Table 4]. Table 4 Determination of precision Accuracy (recovery test) Accuracy of the method was studied by recovery experiments. The recovery experiments were performed by adding known amounts of the drugs in powdered tablets. The recovery was performed at three levels, 80, 100, and 120% of lafutidine standard concentration. The recovery samples were prepared in afore mentioned procedure. Three samples were prepared for each recovery level. The solutions were then analyzed, and the percentage recoveries were calculated from the calibration curve. The recovery values for lafutidine ranged from 100.1 �� 0.07611% [Table 2, Figure 4].
Figure 4 Overlay UV spectra of lafutidine standard and test tablet from 200-400 nm Limit of detection and limit of quantification The Limit of detection (LOD) and limit of quantification (LOQ) of lafutidine were determined by using standard deviation of the response and slope approach as defined in ICH guidelines. The LOD and LOQ for lafutidine are described in Table 3. Determination of active ingredients in tablets The validated method was applied for the determination of lafutidine in tablets (six tablets were assayed and the results in Table 2 indicate that the amount of drug in tablet samples met with requirements (98%�C102% of the label claim). CONCLUSION The developed UV spectrophotometric method was found to be rapid, simple, inexpensive, reproducible, and applicable over a wide concentration range with high precision and accuracy.
The method was validated as per the guidelines laid by ICH. The results of the validated tests were found to be satisfactory and therefore this method can be applied successfully for routine quality control analysis of lafutidine in bulk and pharmaceutical formulation. ACKNOWLEDGMENT We would like to thank Mr. Rambhau Moze Hon��ble President, Genba Sopanrao Moze trust for his kind support. Footnotes Source of Support: Nil Conflict of Interest: None declared.
In this edition of Pharmaceutical Methods, I am impressed by the number of articles that use as their means of analysis Liquid Chromatography with UV/Diode Array Detection (DAD). Five or more years ago, the predictions were that all such UV/DAD would be by now replaced by Liquid Chromatography�CMass Spectrometry (LC�CMS) technologies.
LC�CMS technologies were heralded as infallible as they are highly sensitive and selective even in the presence of multiple compounds within very complex matrices, are capable of finding related analogues and isomers, Cilengitide are high mass accuracy technologies that could discern between isobaric compounds or between co-eluting compounds, and their ability is seemingly endless.