caeruleus (Caer_4537, Caer_1186, Caer_4536, Caer_1187,

caeruleus (Caer_4537, Caer_1186, Caer_4536, Caer_1187, Belinostat Caer_4538, Caer_1188, Caer_4539, Caer_4530, Caer_4535). But only one gene, encoding a phosphopantetheinyl transferase component of a siderophore synthetase, is associated with siderophore biosynthesis (Caer_3105). As it was isolated from a biofilm and a siderophore-transport associated genes were present, we presume that P. caeruleus DSM 24564T is utilizing siderophores, which are produced by other ambient bacteria [65]. The phylogenetic tree of the 16S rRNA gene analysis (Figure 1) with intermingled Phaeobacter and Leisingera species indicates that the classification of P. caeruleus DSM 24564T might need to be reconsidered. Hence, we conducted a preliminary phylogenomic analysis using GGDC [66-68] and the draft genomes of the type strains of the other Leisingera and Phaeobacter species.

The results shown in Table 7 indicate that the DNA-DNA hybridization (DDH) similarities calculated in silico for P. caeruleus DSM 24564T compared to other Phaeobacter species are, in general, not higher than those to Leisingera species. Although, the highest value by far was obtained for P. daeponensis, it was immediately followed by L. aquimarina and L. methylohalidivorans, which is in accordance with Figure 1. Table 7 DDH similarities between P. caeruleus DSM 24564T and the other Phaeobacter and Leisingera species (with genome-sequenced type strains) calculated in silico with the GGDC server version 2.0 [66]*. Acknowledgements The authors would like to gratefully acknowledge the assistance of Iliana Schr?der for growing P.

caeruleus cultures and Evelyne-Marie Brambilla for DNA extraction and quality control (both at the DSMZ). The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under contract No. DE-AC02-05CH11231; the work conducted by the members of the Roseobacter consortium was supported by the German Research Foundation (DFG) Transregio-SFB 51. We also thank the European Commission which supported phenotyping via the Microme project 222886 within the Framework 7 program.
Dehalobacter restrictus strain PER-K23 (DSM 9455), is the type strain of the species Dehalobacter restrictus [1]. Currently two pure cultures of D. restrictus have been described, namely D. restrictus strains PER-K23 and TEA [1,2].

We publish here the first full genome of a pure culture within the genus Dehalobacter and a preliminary comparison with a previously obtained metagenome from a co-culture containing Dehalobacter sp. strain E1 and Sedimentibacter sp [3]. Organohalide respiration GSK-3 (OHR) is considered as a key process in bioremediation of sites contaminated with organohalides such as tetrachloroethene (PCE) and trichloroethene (TCE), leading to a great interest in understanding the physiology and metabolism of organohalide respiring bacteria (OHRB).

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