In the current study, subjects ingested either 3000mg of AAKG or placebo prior to measures of upper and lower body 1RM strength and TLV. One week later, subjects ingested the other supplement and performed the same exercise protocol. A one-week interval was utilized to ensure muscle recovery and allow clearance of the supplement from the body. In order to investigate the ergogenic benefits of acute AAKG on exercise performance the following dependent measures were obtained: HR, 1RM strength and TLV for upper and lower body. Supplementation Participants arrived at the lab and were asked about their activity level for the preceding 48 hours. Upon clearance and following a 5 minute rest,

the participant MG-132 clinical trial ingested either a serving of commercially available

CBL-0137 mouse AAKG (Healthwatchers DE Inc., Bohemia, NY) or a placebo composed of microcrystalline cellulose (Apotheca Inc., Woodbine, IA) with 300ml of water. The placebo was similar in color, size, and texture to the supplement. The selected dose [26] and the timing of supplementation was based on prior research which reported plasma arginine concentrations peaked approximately 60 minutes following oral ingestion of AAKG [27]. Because of budgetary constraints, the ingredients of the supplement were not GSK690693 confirmed via an independent laboratory analysis, and consequently, quality control could be a confounding factor. Exercise protocol The subjects then rested quietly for 45 minutes following the ingestion of the supplement. Next, subjects warmed up on an upright stationary bike (Life Fitness, Brunswick Corporation,

Lake Fores, IL) for 5 minutes. Then, subjects completed two warm-up sets of 1012 repetitions on the standard barbell bench press (Magnum D78, Magnum Fitness Systems, South Milwaukee, WI) with a 61.2kg mass. In order to determine each subjects 1RM on the bench press, a trained technician determined a beginning resistance for the subject to perform their first 1RM D-malate dehydrogenase trial. One-repetition maximum was then determined by increasing mass in 4.5 to 9.1kg increments relative to the subjects ability to lift the first weight. The 1RM was obtained in three to six sets for all subjects. The accepted 1RM was defined as the ability of the subject to complete a full repetition without assistance. Following a three minute rest period, 60% of 1RM was placed on the standard barbell bench press and each subject completed as many repetitions as possible until failure occurred. Failure was defined as the inability to complete a full repetition without assistance. Total load volume for the upper body was calculated by multiplying the 60% of the 1RM by the number of repetitions to failure. Following a five minute rest period, subjects performed two warm-up sets (1215 repetitions) of leg press on a Cybex 45 plate loaded leg press (Cybex Inc., Medway, MA) at a load of 82kg.