To obtain a phylogenetic relationship between the various phylotypes, one representative member of each phylotype was selected. To determine if the number of clones analyzed in lab-reared and field- adapted adults were representative for the each bacterial community, a table was made in which each OTU was listed as many times as its observed frequency. Rarefaction curve was generated by plotting the number of OTUs observed against number of sequences sampled [55]. Acknowledgements This work was supported by research grant from the ‘Core Budget’ of “”International selleck kinase inhibitor Centre for Genetic Engineering
and Biotechnology”" (ICGEB), New Delhi, India. Research fellows AR and AS were supported through grants awarded by “”Department of Biotechnology”" (DBT), New Delhi, India. Electronic supplementary material Additional file 1: Antibiotic sensitivity assay of microbial strains isolated from A. stephensi midgut. The data provided represents the antibiotic response of strains isolated from A. stephensi midgut against selected class of antibiotics. (DOC 88 KB) References 1. Hedges LM, Brownlie JC, O’Neill SL, Johnson KN:Wolbachia and virus protection in insects. Science 2008, 322:702.PubMedCrossRef Go6983 2. McMeniman CJ, Lane RV, Cass BN, Fong AWC, Sidhu M, Wang
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