This was achieved by adopting the optimal experimental condition ascertained in this study, which is specified as 7 mJ laser energy, 1.3 kPa helium pressure, and 50 mu s measurement window, and which was found to result in consistent D emission enhancement. Employing these operational parameters, a linear calibration line exhibiting a zero intercept was obtained from zircaloy-4 samples doped with various concentrations of D impurity, regarded as surrogates for H impurity. An additional measurement also yielded a detection limit of about 10 mu g/g for D impurity, well below the acceptable threshold of damaging H concentration in zircaloy. Each of these
measurements was found Selleckchem SC79 to produce a crater size of only 25
mu m in diameter, promising its application for performing less-destructive measurements. The result of this study has thus paved the way for conducting a further experiment with hydrogen-doped zircaloy samples and the further technical development of a three-dimensional quantitative micro-analysis of detrimental hydrogen impurity in zircaloy vessels used AZD5153 in nuclear power plants. (C) 2011 American Institute of Physics. [doi:10.1063/1.3631776]“
“Background: Diverse growth factors secreted from human adipocyte-derived stem cells (hASCs) that support or manage adjacent cells have been studied for therapeutic potentials to a variety of pathological models. However, senescent growth arrest in hASCs during in vitro culture and subsequent defective differentiation potential, CHIR-99021 solubility dmso have been technical barriers to further genetic modification of hASCs for functional improvement.
Objective: We investigated the feasibility of long-term hASC culture to enhance their therapeutic use.
Methods: We used
a MYC variant to generate hASCs expressing v-myc and determined their growth potential and growth factor secretion profile. We further introduced an AICT variant to generate constitutively active (CA)-Akt/v-myc hASCs. Finally, we tested the ability of promoting wound healing of medium conditioned with CA-Akt/v-myc hASCs.
Results: The v-myc hASCs actively proliferated longer than control hASCs. Increased secretion of vascular endothelial growth factor (VEGF) by v-myc hASCs promoted the migration potential of hASCs and vasculogenesis in co-cultured endothelial cells. Additional genetic modification of v-myc hASCs using CA-Akt further increased VEGF secretion. In addition, injection of CA-Akt/v-myc hASCs-CM into wound-mice model promoted wound healing compared to normal hASCs-CM.
Conclusion: Genetic modification of hASCs to stimulate secretion of growth factors is a novel strategy to maximize their paracrine effect and improve their therapeutic potential. (C) 2012 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.