The expression levels of two proteins (Gpd, spot 26; and RfbC, sp

The expression levels of two proteins (Gpd, spot 26; and RfbC, spot 42) however were not impacted following exposure to 3.6% Oxgall (absolute value of variation factor r ≤ 1.5), suggesting a minor role for these in the bile tolerance process of the considered L. plantarum strains. Discussion This www.selleckchem.com/products/E7080.html paper reports the application of 2-DE and MS analysis to investigate LAB proteins that are key in the bile tolerance process, a major factor when it comes to probiotics adaptation to the GI tract. Although

2-DE has known limitations and only explores part of bacterial proteomes as compared to other IWR-1 nmr gel-less analyses [31], it is a widely used and affordable technique which proved to be valuable in discriminating strains according to their bacterial features [22–25]. With regard to probiotic research, two previous studies used a similar approach to explore adhesion properties of L. plantarum [12] and B. longum [26]. However, this is the first time that an attempt is made towards getting a broad picture of bile tolerance at the species level rather than focusing on a single strain. L. plantarum, a versatile

species with marketed probiotic strains, was chosen as a model for this study. An in vitro test was used to assess bile tolerance of nine strains, including L. plantarum Selleck Milciclib 299 V, a probiotic with outstanding bile resistance properties [32]. These properties were confirmed in our study, as this strain showed the best ability to grow in bile supplemented Liothyronine Sodium culture broths. Considerable variations in growth rates were observed between strains, with the highest effect of bile on L. plantarum LC 56, which is in accordance with previous reports showing a strain-specific behavior of LAB with regard to bile tolerance [33, 34]. Strains LC 56 (weak bile tolerance), LC 804 (intermediate

bile tolerance) and 299 V (strong bile tolerance) were selected for the proteomic investigation. For that purpose, we focused on the whole cell proteomes, since the ability of an organism to tolerate bile may require a wide array of proteins implicated in either membrane- or cytosol-based functions and mechanisms [27]. The differentially expressed proteins among the three selected strains cultured in standard conditions all appeared to be encoded by highly conserved genes in the L. plantarum species. These core-genome proteins are of great interest in the search for bacterial biomarkers as their relative abundance is likely to be assessed for any L. plantarum strain. In our case, 10 proteins displayed increasing levels of expression from the sensitive strain (LC 56) to the resistant one (299 V), suggesting a positive correlation of these proteins with bile resistance.

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