Proteasome inhibitor structure of unsaturated fatty Ttigten lactone electrophile

Re largely used to treat various proteasome inhibitor inflammatory neurological disorders. Z ligustilide has a structure of unsaturated fatty Ttigten lactone electrophile, which reacts with several cysteine residues in Keap1 protein. Alkylation of specific cysteine residues blocked with the formation of complex Keap1 Nrf2, Nrf2 and is translocated into the nucleus, sen the antioxidant response auszul. In fact, Z ligustilide and phthalides associated recently shown that cellular Re resistance to oxidative Zellsch Autocompletion and occurrence of stem cell proliferation in rats mesenchylmal. Recent studies have au Addition show that Z-ligustilide lipopolysaccharide-induced inflammatory reaction is inhibited in prime Ren rat microglia. Along this line, we have found that senkyunolide H and I the cells against H2O2-induced cell death protected by induction of antioxidant enzymes such as HO-1. Among the various inducers 1 HO, Z ligustilide a small molecule, the lipophilic, the blood-brain barrier. We have also shown that Z ligustilide k Nnte dopamine cells from oxidative Sch Termination by the activation of SAR signals to protect and to induce cell-mediated depletion of reduced GSH. Thus, we assumed that Z ligustilide k nnte Cellular Ren resistance of dopaminergic neurons of the cytotoxicity t of dopamine by regulating the intracellular Improve Ren redox status and decreases the level of GSH. In this study we have determined the effect of Z ligustilide toxicity t of dopamine in dopaminergic PC12 cells. To Ren, the molecular potential to kl, We examined the effect of dopamine Z ligustilide and in combination on the intracellular Re formation of ROS, reduced GSH levels and apoptotic Streptozotocin functions. The results of this study is that Public at concerns about the combined use of drugs Nahrungserg Supplements you with L dopa in the treatment of Parkinson’s disease.
Materials and methods Chemicals Z ligustilide was bought in Hong Kong Jockey Club Institute of Chinese Medicine with a purity of over 98%. Dopamine and other chemicals were obtained from Sigma Aldrich Co., unless otherwise indicated. Cell culture All cell lines were used in this study from the American Type Culture Collection cell is obtained. Rat Ph Ochromozytom PC12 cells maintained in Dulbecco’s modified Eagle medium containing 10% horse serum erg Complements, 5% f Fetal K Calf serum, 1% penicillin / streptomycin on collagen I coated dishes. The man of SH human neuroblastoma cells, HepG2 hepatocellular liver Ren cancer SY5Y human epithelial carcinoma HeLa cells and human breast adenocarcinoma MCF-7 cells were cultured in DMEM erg complements With 10% FBS, 1% penicillin / streptomycin. Prostate cancer in humans PC3 cells were cultured in RPMI-1640 supplemented with 10% FBS, 1% penicillin / streptomycin. All cell lines were cultured at 37 ° C in a 5% CO 2 atmosphere re of 95% air. Measuring the ability Lebensf Of the cells Lebensf Ability of the cells was examined by a standard color test with diphenyltetrazolium 3 2.5 as described above. Briefly, at the end of the drug Sen treatment, the cell culture medium was replaced solution with 0.5 mg / ml MTT-L Solution in phosphate-buffered salt solutions. The cells were incubated at 37 ° C for 4 hours. Thereafter, the MTT-L Solution coated, removed, and the result was formazan by an equal volume of dimethyl sulfoxide dissolved St at 37 ° C.

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