Plasma adrenocorticotrophic hormone (ACTH, ALPCO Diagnostics, Salem, NH), C-reactive protein (CRP, Diagnostic Systems Laboratory, Webster, TX), and malondialdehyde (MDA, Cell Biolabs Inc., San Diego, CA) C646 supplier concentrations were assayed in duplicate via ELISA. Determination of serum immunoreactivity values was made using a SpectraMax340 Spectrophotometer (Molecular Devices, Sunnyvale, CA). Serum creatine kinase (CK) concentrations were determined at 340 nm on a spectrophotometer (Pointe Scientific, Inc, Canton,

MI). Plasma glutamine, glucose and lactate (La-) concentrations were determined in duplicate with an automated analyzer (Analox GM7 enzymatic metabolite analyzer, Analox Instruments USA, Lunenburg, MA). Plasma sodium and potassium concentrations were assessed via ion-selective electrodes (EasyElectrolyte, selleck chemicals llc Medica, Bedford, MA). To eliminate inter-assay variance, all samples were analyzed in the same assay run. Intra-assay variance for all assays was < 10%. Plasma volume shifts following the workout were calculated using the formula of Dill & Costill [19]. Statistical Analysis All data were assessed and met assumptions NVP-BSK805 datasheet for normal distribution, homogeneity of variance, and sample independence. Statistical evaluation of performance, hormonal and biochemical changes were analyzed using a repeated measures analysis of variance (ANOVA). In the event of

a significant F- ratio, LSD post-hoc tests were used for pairwise comparisons. Prior to the ANOVA, Plasma volume shifts, performance comparisons, and area under the curve (AUC) calculated

using standard trapezoidal technique were analyzed using a One-Way ANOVA. Significance was accepted at an MYO10 alpha level of p ≤ 0.05. All data are reported as mean ± SD. Results Usg (1.026 ± 0.004), Uosm (813 ± 299 mOsm) and Posm (297.0 ± 4.6 mOsm) were similar for all trials at DHY. These results reflected the overnight fasting and exercise-induced dehydration performed during prior to each trial. Plasma glutamine concentrations were significantly higher for all groups at RHY and IP compared to BL (p = 0.002 and p = 0.000, respectively) and DHY (p = 0.001 and p = 0.000, respectively) (Figure 1). [Glutamine] for T5 were significantly higher at RHY and IP than T2 – T4. AUC analysis showed significantly greater [glutamine] for T5 at all time points compared to the other experimental trials (see Figure 2). Figure 1 Plasma Glutamine Concentrations. There was a significant main effect for trial between T2 and T5. # = significant main effect for time versus BL and DHY; a = significantly different from T2, T3, and T4. Figure 2 AUC Glutamine. * = Significantly different from T2 Time to exhaustion was significantly reduced during T2 than at any other experimental trial (see Figure 3). When examining Δ performance (difference between each experimental trial and T1), time to exhaustion was significantly greater during T4 (130.2 ± 340.2 sec) and T5 (157.4 ± 263.