HBO2 therapy to Atients exposed, as in decompression sickness, carbon monoxide poisoning and smoke inhalation, gas gangrene, acute Isch Chemistry traumatic wound healing, necrotizing soft tissue infections, radiation Gewebesch the compromised skin grafts, and br thermal lures. Zus Tzlich to the above directions clinics, which were approved by the Food and Drug Administration, there are several other diseases, for the positive effects HBO2 Including product Lich improving the cognitive and neurological function after cerebral ish Would have chemistry. Previous studies have shown that nitrous oxide can k for medical gases A angstl induce Send effect, as in animals depends h Of nitric oxide. Since our evidence that HBO2 treatment may also NO production is provided in the brain, we investigated whether HBO2 can arouse as an anxiolytic in animals. Animals NIH Swiss male pattern method, 20 25 g of K Body weight were purchased from Harlan Laboratories. The experiments were carried out by an Animal Care Review Board and approved in accordance with the use of authorized and carried out in accordance with the Guide for the Care and Use of Laboratory Animals, 8th Edition. The Mice were housed five per K Fig in the Wegner Hall Vivarium with ad libitum access to food and water. Dark cycle of 12 h under standard conditions: the PARP Inhibitor plant is maintained over a 12 h light. The Mice were kept in the waiting room for at least four days after arrival at the facility. Each animal was used once and thrown away. Rockford, IL). An acrylic divider with a 75 mm 75 mm He × Opening the floor divided the box In a bright and a dark compartment compartment. The W Walls of light and dark chambers were black and white with acrylic, Respectively.
Conduct observations and evaluations were carried out in the rule 1000-1400 h. In all experiments, the subject was by the light of the two R Lead of 40 W fluorescent white Em light mounted directly illuminated above 180 mm. In this paradigm, the animals were individually placed in the middle chamber of the box They then observed the light for 5 min. The time spent in the room with the light of Bo It was recorded for each mouse. A mouse was considered in the new area in which all four legs to have crossed the threshold into the compartment. An increase Issued increase the amount of time in the chamber of the lamp is a sign of an ALK anxiolytic effect of the drug. Exposure to hyperbaric oxygen chamber K Five provisional M usen Were placed in a pressure chamber Research B 11 as described above. The room was with 100% O2 U.S.P. ventilated at a rate of 10 l / min. The pressure in the cylinder chamber acrylic min was increased by more than 2 to the desired pressure Ht and for 60 min. The M could Mice spontaneously breathing w During HBO2 treatment. After completion of HBO2 exposure were min Mice decompressed over 2. Groups of control The Mice were treated with compressed air st mt Through the chamber exposed at 1.0 ATA and held for 60 min. S expertise in the well R time, various groups of M Nozzles Angstzust Walls at various time intervals after the end of HBO2 reviewed the treatment. Drugs The following drugs were used in this study: fl.