It also differs from other North American species of the genus by additional features such as the body size, the length of spicules, or the length of the female tail. Syngnathinema chitwoodi (a single subgravid female) differs from the only other congener, Syngnathinema californiense Moravec, Spangenberg and Frasca, 2001, a parasite of the circulatory system of the pipefish in California and British Columbia, mainly in that the posterior end of the muscular esophagus is not submerged into the anterior end of

the glandular esophagus. Previous reports of Camallanus oxycephalus Ward and Magath, 1917 in P. spathula may be misidentifications of C. polyodontis.”
“17 alpha-hydroxylase/17,20-lyase deficiency (17OHD) is a rare autosomal recessive genetic disease that is characterized by low-renin hypertension, hypokalemia, and abnormal development of the genitalia. Mutations in the CYP17A1 Pevonedistat in vitro gene account for this disease. We aim

to investigate the CYP17A1 mutation and analyze its possible influence on phenotype in a Chinese patient with 17OHD. Steroid hormones were assayed. The 8 exons of the CYP17A1 gene were amplified and directly sequenced. Wild-type and mutant GSK1210151A chemical structure CYP17A1 cDNA were cloned into pcDNA3.1 expression vectors and transfected into 2931 cells. Finally, 17-hydroxylase and 17,20-lyase activity were detected by using progesterone and 17-hydroxypregnenolone as the substrates. A novel missense mutation c.716 G>A located in exon 4 that

changed the amino acid from arginine to glutamine (R239Q) was discovered in the patient. AZD8186 mw Steric model analysis of CYP17A1 showed that R239Q changed the local structure and the electrostatic potential. Functional study indicated that the R239Q mutant caused the complete loss of both 17 alpha-hydroxylase and 17,20-lyase activities. Our study expanded the CYP17A1 mutation spectrum. With a functional study, we confirmed that the novel mutation caused the complete loss of both 17 alpha-hydroxylase and 17,20-lyase activities. (Translational Research 2013;161:44-49)”
“Lemon balm (Melissa officinalis L) is one of the most important herbs known for curing Alzheimer’s disease. In this study, the effect of time and temperature on the quality of essential oils was investigated. The essential oil of air-dried samples was obtained by hydro-distillation and was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Changes in essential oil compositions were detected during storage for four months in a refrigerator (4 C), a freezer (-20 C), and at room temperature. The results indicated that, at room temperature, the proportions of compounds with lower boiling temperatures such as citronellal (25.8-12.6%), neral (18.9-4.0%) and geranial (27.0-4.6%) were decreased. Furthermore, the oil compositions showed the least alterations and M.