In symbiotic conditions, expression of these

genes showed a general trend to a down-regulation in whole animals (37/43) and ovaries (31/44). On the contrary, 30 genes among 37 are over-expressed in immune tissues (Table 4 and Additional File 5: Expression profiles of genes studied in whole animals, ovaries, and immune tissues of A. vulgare). Significant differential expressions in whole animals and ovaries were recorded for 16 genes, 12 of them were down-regulated and 4 up-regulated (Table 4). No significant differential expression see more was detected in immune tissues. Three genes involved in pathogen recognition, the C-type lectin 1, C-type lectin 2, and the C-type lectin 3 genes were differentially expressed. The C-type lectin 1 was up-regulated in ovaries whereas the C-type lectin 2 was down-regulated in the same tissue. Finally, the C-type lectin 3 was down-regulated in the whole animals. Three genes encoding AMPs were down-regulated: The armadillidin and the {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| i-type lyzozyme genes in whole animals and the crustin3 gene in both whole animals and

ovaries. One serine protease gene, the masquerade-like B, was also under-expressed in whole animals. Three genes involved in detoxification, the peroxiredoxin A and C and glutathione peroxidase, were down-regulated in ovaries whereas the thioredoxin A was up-regulated in the same tissue. In the autophagy pathway, two genes, atg7 and atg12, were under-expressed in ovaries. Among genes involved in stress response, the ferritin A and C genes were over-expressed in ovaries. Discussion The different EST libraries generated in this study constitute the first reference transcriptome ever obtained in the Methane monooxygenase Isopoda group [51]. Among crustaceans, only the Daphnia

pulex (Branchiopoda, Cladocera) genome was recently published [52] and some EST libraries were constructed from a shrimp, a crayfish, and a porcelain crab (Malacostraca, Decapoda) [53–57]. Another EST database was obtained in the marine isopod Limnoria quadripunctata, but it concerned only the hepatopancreas [58]. Thus, our result represents the eighth largest sequencing effort for any crustacean, behind the cladoceran Da. pulex and the decapods Litopenaeus vannamei and Petrolisthes cinctipes, and the sixth EST data set for any Malacostraca species [51, 57]. Few A. vulgare unigenes present similarities with crustacean ESTs. This could be in part explained by the phylogenetic distance between isopods and the crustaceans from which EST libraries or genomics data are available. However, the overlapping between libraries was low, suggesting that the sequencing effort should be increased. The present work allowed us to identify the first immune gene repertoire from a terrestrial crustacean.