In combined confounder-adjusted models (model 1) for girls, there were selleck chemicals greater TPCA-1 clinical trial paternal smoking associations with TBLH BMC, BA and BMD and spine BMD compared with those for maternal

smoking, whilst maternal associations were larger than paternal associations with spine BMC and BA. There were no strong associations of paternal smoking with bone outcomes in boys. On additional adjustment for the child’s birth weight and gestational age (model 2), there were increases in maternal associations, whilst paternal associations did not change. In boys, maternal smoking in all trimesters was positively associated with TBLH BMC, BA and BMD after adjustment for birth weight and gestational age. In fully adjusted models including offspring height and weight at age 9.9 years (model 3), all maternal relationships attenuated to the null, although a weak association remained with spine BA in girls. Paternal associations were similarly attenuated, and although evidence remained of an association with TBLH BA, this weakened in combined models. There were no associations between parental smoking during pregnancy and TBLH or spine

ABMC, except for a weak positive association between paternal smoking and spine ABMC in girls. Table 2 Sex-specific selleck screening library associations of maternal and paternal smoking with total body less head bone outcomes at age 9.9 years

in multiple imputation analysis (boys N = 3,530; girls N = 3,591)   Mean difference 95% CI P value Mean difference 95% CI P value Mean difference 95% CI P value Boys TBLH BMC (SD score: 1 SD = 174.6 g) TBLH BA (SD score: 1 SD = 154.9 cm2) TBLH BMD (SD score: 1 SD = 0.053 g/cm2) Maternal smoking in any trimester Model Tau-protein kinase 1 0.01 −0.07–0.09 0.767 0.00 −0.08–0.08 0.992 0.04 −0.05–0.12 0.419 Model 2 0.05 −0.03–0.14 0.186 0.05 −0.03–0.13 0.232 0.06 −0.03–0.15 0.177 Model 3 0.00 −0.05–0.04 0.885 −0.01 −0.04–0.03 0.736 0.01 −0.06–0.08 0.752 Maternal smoking in all trimesters Model 1 0.07 −0.04–0.17 0.200 0.05 −0.05–0.15 0.356 0.10 −0.01–0.21 0.086 Model 2 0.13 0.02–0.23 0.016 0.12 0.01–0.22 0.025 0.13 0.02–0.24 0.020 Model 3 0.00 −0.06–0.05 0.877 −0.02 −0.06–0.03 0.482 0.03 −0.06–0.12 0.523 Paternal smoking Model 1 0.02 −0.05–0.10 0.519 0.03 −0.04–0.10 0.405 0.01 −0.07–0.08 0.887 Model 2 0.03 −0.04–0.10 0.425 0.04 −0.03–0.11 0.305 0.01 −0.07–0.08 0.833 Model 3 −0.02 −0.05–0.02 0.357 −0.01 −0.04–0.02 0.581 −0.03 −0.09–0.03 0.313 Combined models Model 1 Maternal smokinga 0.01 −0.08–0.09 0.830 −0.01 −0.09–0.08 0.888 0.04 −0.05–0.13 0.396 Paternal smoking 0.03 −0.04–0.