Hypercholesterolemia and elevation of plasma LDL in this model is due to heavy proteinuria which is caused by glomerulosclerosis. Table 1 General data in the 5/6 nephrectomized (CRF), and sham-operated control (CTL)

rats   CTL CRF Body weight 12 weeks (g) 459.80 ± 21 411.7 ± 55.3 Systolic blood pressure 12 weeks (mmHg) 123.5 ± 13 168.8 ± 2.8** 24 h urine protein 12 weeks (g/day) 6.7 ± 1.2 80.3 ± 7.3** Plasma urea nitrogen (mg/dl) 25.3 ± 2.0 60.0 ± 16.4*** Plasma creatinine (mg/dl) 0.50 ± 0.14 2.2 ± 1.5* Plasma total cholesterol (mg/dl) 73.6 ± 8.6 221.2 ± 2.5*** Plasma triglyceride (mg/dl) 45.8 ± 18.2 99.7 ± 3.5** Plasma LDL cholesterol (mg/dl) 18.1 ± 5.3 95.0 ± 14.0*** N = 6 in each group. Data are mean ± SD, * P < 0.05, ** 0.01, *** 0.001 LPL and GPIHBP1 data Data are depicted in Figs. 1, 2, and 3. Compared with the sham-operated control Pictilisib mouse rats, the CRF group exhibited a significant reduction of LPL mRNA abundance in both skeletal muscle Wortmannin in vitro and adipose https://www.selleckchem.com/products/ly333531.html tissue (P < 0.001). Likewise LPL protein abundance was significantly reduced in skeletal muscle (P = 0.0003), myocardium (P = 0.035) as well as subcutaneous (P = 0.034) and visceral (P = 0.0001) adipose tissues of the CRF rats. The reductions in LPL mRNA and protein abundance in the skeletal muscle and adipose tissue in the CRF animals were accompanied by parallel reductions

of GPIHBP1 mRNA abundance in the tested tissues. Histological examination of the adipose tissue revealed a significant reduction of the size of the adipocytes in the CRF compared to the control group. This observation points to diminished lipid stores in the adipose tissue which is largely mediated by CKD-induced LPL deficiency. Immunohistological examination of the tissues showed a significant reduction of the GPIHBP1 immunostaining in the endothelium of the capillaries in the skeletal muscle,

adipose tissue and myocardium in the CRF animals compared to the corresponding tissues in the control group (Fig. 3). Fossariinae Fig. 1 Bar graphs depicting LPL/beta-actin mRNA ratios and GPIHBP1/beta-actin mRNA ratios in the skeletal muscle and adipose tissues of the CRF and normal control groups. N = 6 in each group, *P < 0.05, **0.01, ***0.001 Fig. 2 Representative Western blot and group data depicting LPL and beta actin protein abundance in the subcutaneous fat (a), visceral fat (b), skeletal muscle (c), and myocardium (d) of the CRF and normal control groups. N = 6 in each group, *P < 0.05, ***0.001 Fig. 3 Representative photomicrographs depicting GPIHBP1 immunostaining of the skeletal muscle, myocardium, and adipose tissue of a CRF and a normal control rat Discussion Until recently the lipolytic process was thought to be primarily controlled by myocytes and adipocytes with the adjacent capillary endothelial cells playing a passive part by hosting this process.