EGR1 was scored using a six point scale. As molarity calculator control samples for STAT6 and EGR1, some possible mimickers of SFT were stained. Endothelial nuclei served as an internal positive control for EGR1. Clinical information, where available, was obtained from the hospital records. Reverse transcriptase Polymerase chain reaction Inhibitors,Modulators,Libraries RNA was extracted from formalin fixed and paraffin embedded tissues using RNA Bee RNA isolation reagent according to standard procedures. RNA quantity and quality were determined by a NanoDrop measurement and subsequently, cDNA synthesis Inhibitors,Modulators,Libraries was performed using Superscript II and random hexamers. Results Clinical data Clinical data are summarized in Table 2. Twenty eight patients were selected, including 10 male and 18 female patients with an age range of 21 84 years.
Tumors arose in the lung and pleura, deep soft tissue of the lower extremities, head and neck, abdomen and pelvis. Other sites were uterus, retroperitoneum, deep soft tissue of the upper extremity and deep soft tissue of the back. Inhibitors,Modulators,Libraries Surgical excision was performed in 28 patients, 1 after prior embolization. Resection margins were positive in 3 patients, negative in 21 patients, and not reported in 4 cases. One patient with positive margins and a subsequent local recurrence received surgery combined with radiotherapy for his recurrent disease and had no evidence of disease at 7 months. The other 2 patients with positive resection margins did not receive post surgical therapy and had no evidence of disease at 17 and 23 months. Recurrences occurred in 4 instances including 3 patients with negative margins and the above mentioned case 16 with positive margins.
One of the 3 patients with negative margins had surgical therapy for several local recurrences and metastatic disease. One patient received surgery Inhibitors,Modulators,Libraries for local recurrence and chemotherapy after discovery of metastatic spread. Fifteen patients had no recurrences, and of 9 patients, data about local recurrences were not known. Metastatic disease occurred in 3 patients, including case 1 and case 3 mentioned above. One patient received combined radio chemotherapy after discovery of metastases. Sixteen patients showed no metastatic disease and of the remaining 9 cases, data were not known. Two patients died of metastatic disease 13 months and 52 years after the initial diagnosis. In case 1, recurrences and metastases were histologically proven.
Currently, 16 patients have no evidence of disease with a Inhibitors,Modulators,Libraries median follow up of 29. 5 months, including 3 patients with long term disease free follow up. Ten patients were lost to follow up or no follow up data could be obtained. Gross findings Tumor size ranged from 1. 6 17 cm. Grossly, the tumors had a nodular configuration with a coarse white grayish cut surface. Myxoid areas http://www.selleckchem.com/products/Abiraterone.html were apparent in some cases.