Acknowledgements This work was supported by Grants No.09320503600 and No.10PJ1404900 from Shanghai Municipal Science

and Technology Commission, and Grants No.B-9500-10-0004 from Shanghai Municipal Education Commission, No.QXJK201207 from Shanghai Meteorological Bureau, and No.31271830 from National Natural Science Foundation of China. References 1. Wozniak RA, Waldor MK: Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow. Nat Rev Microbiol 2010, 8:552–563.PubMedCrossRef 2. Gogarten JP, Townsend JP: Horizontal gene transfer, genome innovation and evolution. Nat Rev Microbiol 2005, 3:679–687.PubMedCrossRef 3. Nakayama K, Yamashita A, Kurokawa K, Morimoto T, Ogawa M, Fukuhara M, Urakami H: The whole-genome sequencing of the obligate intracellular bacterium orientia tsutsugamushi revealed massive gene amplification during P505-15 reductive genome evolution. DNA Res 2008, 15:185–199.PubMedCrossRef 4. Burrus V, Waldor MK: Shaping bacterial genomes with integrative and conjugative elements. Res Microbiol 2004, 155:376–386.PubMedCrossRef 5. Scott JR, Churchward GG: Conjugative transposition. Annu Rev Microbiol 1995, 49:367–397.PubMedCrossRef 6. Whittle Quisinostat mw G, GS-1101 in vivo Shoemaker NB, Salyers AA: The role of Bacteroides conjugative transposons in the dissemination of antibiotic resistance genes. Cell Mol Life Sci 2002, 59:2044–2054.PubMedCrossRef 7. Burrus V, Marrero J, Waldor

MK: The current ICE

age: biology and evolution of SXT-related integrating conjugative elements. Plasmid 2006, 55:173–183.PubMedCrossRef 8. Bani S, Mastromarino PN, Ceccarelli D, Van AL, Salvia AM, Viet QTN, Hai DH, Bacciu D, Cappuccinelli P, Colombo MM: Molecular characterization of ICE Vch VieO and its disappearance in Vibrio cholerae O1 strains isolated in 2003 in Vietnam. FEMS Microbiol Lett 2007, 266:42–48.PubMedCrossRef Megestrol Acetate 9. Taviani E, Ceccarelli D, Lazaro N, Bani S, Cappuccinelli P, Colwell RR, Colombo MM: Environmental Vibrio spp., isolated in Mozambique, contain a polymorphic group of integrative conjugative elements and class1 integrons. FEMS Microbiol Ecol 2008, 64:45–54.PubMedCrossRef 10. Rodríguez-Blanco A, Lemos ML, Osorio CR: Integrating conjugative elements as vectors of antibiotic, mercury, and quaternary ammonium compound resistance in marine aquaculture environments. Antimicrob Agents Chemother 2012, 56:2619–2626.PubMedCrossRef 11. Thompson FL, Klose KE, AVIB Group: Vibrio 2005: the first international conference on the biology of vibrios. J Bacteriol 2006, 188:4592–4596.PubMedCrossRef 12. Pruss A, Havelaar A: The global burden of disease study and applications in water, sanitation and hygiene. In Water quality: guidelines, standards and health. Edited by: Fewtrell L, Bartram J. London: IWA Publishing; 2001:43–59. 13. Wilcox BA, Colwell RR: Emerging and reemerging infectious diseases: biocomplexity as an interdisciplinary paradigm.