A-966492 onverting enzyme is known, were involved in the transactivation. To test whether the induction of hBD 3 by transactivation of EGFR was caused, was incubated ex vivo wounded skin with an inhibitor of TACE, tumor necrosis factor rotease inhibitor first A TAPI inhibited the expression of hBD third In contrast, inhibitors of serine proteases or cysteine proteases no influence on the expression of hBD 3 in injured skin. To identify the ligand for EGFR expression hBD 3, wounded skin was blocking Antique Rpern against the EGFR ligands TGF incubated e HB EGF.
These two growth factors, EGFR ligands are on the st Strongest expression in the skin, and they are the most potent inducers of hBD third Blocking Antique Body against HB-EGF but not TGF artially inhibited the mRNA expression of hBD-third To PHA-739358 the R To check the HB EGF in the induction of hBD 3, wounded skin was incubated with CRM197 which inhibits a non-toxic analog of diphtheria toxin, which specifically bind to and release of membrane-bound HB EGF, but does not inhibit the effect of EGF L soluble HB or any of the other EGFR ligands. The addition of CRM197 inhibited the induction of the mRNA hBD 3, and the two TAPI 1 and CRM197 also inhibited the expression of peptides HBD 3, as detected by IHC. Thus, the Erh Increase the concentration of hBD 3 breach in the skin of HB EGF wounded skin by transactivation of EGFR mediated. After injury approximately 50 ng hBD 3 were detected in the extract of 0.15 cm2 of the skin over 4 days. Assuming that the thickness of the epidermis about 0.
25 mm betr Gt, so that a concentration of 3 about 13 hBD �g / ml, since the most intense F Staining for hBD 3 to the R Change of the wounded and in the upper layers of the epidermis was found, the local concentrations of hBD in these three areas likely to be much h higher than the concentration in the entire epidermis. Served as the shops PROTECTED concentration of 3 whole in hBD epidermis required on the concentration of hBD three Streptococcus pyogenes skin t Th important pathogens, we investigated whether activation of EGFR k Nnte the entire activity T to obtained Hen antibacterial the epidermis. Organotypic cultures were stimulated with epidermal TGF round then extracted for analysis in the antibacterial tests. Epidermis contains Lt prominent antibacterial activity of t against Escherichia coli.
To test the efficiency of extraction of SAP from the epidermis, we examined the activity t of epidermal extracts against E. coli and found, as expected, the prime Activity re t unstimulated against E. coli in the extracts of two and TGF Stimulated epidermal cultures. In contrast, and consistent with previous findings, extracts from unstimulated epidermal cultures showed no significant antibacterial activity of t against Staphylococcus aureus compared to the buffer control. However, extracts from epidermal cultures by TGF erh Ht ad fa Is significant antibacterial activity of t against S. aureus stimulated with extracts from unstimulated cultures compared epidermal or controlled the buffer. Sun stimulates activation of EGFR in the subsequent induction of SAP in sterile Sch Ending of the antibacterial properties of the epidermis of the skin against a pathogen. Discussion We have assumed that the expression of SAP in the skin can be induced after sterile injury. In fact, we found that injury induces the expression of sterile 3 amps in the human skin, hBD 3, NGAL and SLPI. We have already found