Alpha conidia
9–12 × 2–3.5 μm (x̄SD =10 ± 1 × 3 ± 0.3, n = 30), abundant on alfalfa twigs, aseptate, hyaline, smooth, ovate to ellipsoidal, biguttulate or multiguttulate, base subtruncate. Beta conidia not observed. Cultural characteristics: In dark at 25 °C for 1 wk, colonies on PDA moderate growth rate, 3.8 ± 0.2 mm/day (n = 8), white, aerial SB431542 in vitro mycelium turning to grey at edges of plate, reverse white in centre; stroma produced in 1 wk old culture with abundant conidia. Host range: On Juglans cinerea and Juglans sp. (Juglandaceae) Geographic distribution: Canada (Ontario); USA (Iowa, New York, Pennsylvania, Tennessee). Type material : USA, New York, Greenbush, on branch of Juglans cinerea, (NYS F 468, holotype); Tennessee, Great Smoky Mts National Park, dead wood of Juglans sp., 8 May 2006, L. Vasilyeva (BPI 878472, epitype designated here, ex-epitype culture selleck compound DP0659 = CBS 121004; MBT178536). Additional material examined: CANADA, Ontario, Granton, on dead branches of Juglans sp., July 1898, J. Dearness (BPI 615762, 615766); USA, Iowa, Decorah, on dead branches of Juglans sp., June 1892, E.W.D. Holway (BPI 615761, BPI 615765); Pennsylvania, Bethlehem,
on twigs of Juglans cinerea, 9 June 1922, C.L. Shear 4043, det. F. Petrak (BPI 615764). Notes: Diaporthe bicincta has long paraphyses and larger conidia (9–12× 2–3.5 μm) than D. juglandina on Juglans in Europe. The isolate CBS 121004 was deposited as D. juglandina (Gomes et al. 2013); however, this isolate was originally Go6983 datasheet from the USA (Tennessee) and is here confirmed as D. bicincta based on a morphological comparison with the type and non-type specimens. Diaporthe celastrina Ellis & Barthol., J. Mycol. 8: 173 (1902). Fig. 7d–f Pycnidia on host and alfalfa twigs on WA 200–300 μm diam, globose, embedded in tissue, erumpent at maturity, well developed, black stroma with a 50–150 μm
long necks, often with an off-white, conidial cirrus extruding from ostiole; walls parenchymatous, consisting of 3–4 layers of medium brown textura angularis. Conidiophores 7–21 × 1–2 μm, hyaline, smooth, unbranched, ampulliform, cylindrical. Conidiogenous cells 0.5–1 μm of diam, phialidic, cylindrical, terminal, slightly tapering towards apex. Paraphyses absent. Alpha conidia 9–12 × 2–3.5 μm (x̄±SD =10 ± 0.8 × 2.7 ± 0.3, n = 30) abundant on alfalfa twigs, aseptate, hyaline, smooth, ellipsoidal, biguttulate, multiguttulate, or eguttulate, base subtruncate. Beta conidia not observed. Cultural characteristics: In dark at 25 °C for 1 wk, colonies on PDA fast growing, 5.8 ± 0.2 mm/day (n = 8), white aerial mycelium, reverse white in centre; stroma produced in 1 wk old culture. Host range: On Celastrus scandens (Celastraceae). Geographic distribution: USA (KS, VA). Type materialUSA, Kansas, Clyde, Celastrus scandens, 18 May 1901, E. Bartholomew 2856 (BPI 615293, holotype). USA, on Celastrus scandens, September 1927, L.E. Wehmeyer (BPI 892915, epitype designated here, ex-epitype culture CBS 139.