In our recent work, we cocultured the hippocampal slices from control and seizure animals to visualize what is going on in the brain during epileptogenesis. Even though it must be noted that the brain slice culture system includes reorganization of some neural circuits which are not observed in vivo, it still offers the investigator the opportunity to examine the cellular and molecular mechanisms underlying epileptogenesis-related changes in the neural circuits. With these properties of the slice culture system, in addition to a relatively simpler
experimental manipulation compared to that with in vivo, the use of organotypic slice cultures will this website thus contribute to the discovery of novel therapeutic targets and strategies for preventing the emergence of epileptogenic foci. I thank Dr. Norio Matsuki, Dr. Yuji Ikegaya, and the members
of Laboratory of Chemical Pharmacology (Yaku-Saku Lab) for supporting the projects on experimental febrile seizures. This work was supported by a Grant-in-Aid for Science Research on Young Scientists (B) (No. 19790048) and the Research Foundation for Pharmaceutical Sciences. “
“Hypoxic-ischemic encephalopathy due to AZD2014 neonatal asphyxia is one of the most important causes of delayed neurological development. Prolonged neuronal apoptosis plays an important role in the processes contributing to neuronal degeneration. Docosahexaenoic acid (DHA), a major component of brain membrane phospholipids, prevents neuronal cell apoptosis and plays Leukocyte receptor tyrosine kinase an important role as an anti-oxidant agent. We investigated the neuroprotective and anti-oxidant effects of maternal DHA supplementation during pregnancy in a model of neonatal hypoxic-ischemic encephalopathy. Pregnant rats were randomly assigned
to two experimental groups: a control group or a DHA-enriched diet group. Hypoxic-ischemic encephalopathy was produced by left common carotid artery occlusion and exposure to 8% oxygen for 1.5 h. TUNEL assay, immunohistochemistry for caspase-3 and 8-hydroxy-deoxyguanosine (8-OHdG), and Western blot for caspase-3 were performed at postnatal days 8, 10 and 14. Fatty acid composition of brain was estimated on postnatal day 7. Maternal diet clearly influenced brain fatty acid composition in pups. Numbers of apoptotic neuronal cells and 8-OHdG immunoreactivity were significantly decreased in the DHA-enriched group. Our findings indicate that maternal DHA-enriched diet during pregnancy provides neuroprotection by inhibiting oxidative stress and apoptotic neuronal death. Dietary supplementation of DHA during pregnancy may thus be beneficial in preventing neonatal brain injury. “
“K. E. Funk, R. E. Mrak and J.