5 wt % human serum Androgen Receptor inhibitor albumin (HSA) or 20% dextran solution passed through PU-COOH membranes. The recovery ratios of CD34(+) cells using platelet-poor plasma and platelet-rich plasma were approximately 20% and 30%, respectively, significantly less than the ratio found using 0.5 wt % HSA
solution. Surface-modified membranes having carboxylic acid groups showed a higher recovery ratio of CD34(+) cells than membranes having zwitterionic groups. The effect of carboxylic acid groups on the surface-modified PU membranes was to generate weak interactions by electrostatic repulsion between CD34(+) cells and the membranes because of the negatively charged surfaces of the cells, allowing them to be detached from the membranes and collected in the recovered solution. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 114: 671-679, 2009″
“Bacillus subtilis H27, an isolate from a commercial cheonggukjang product, inhibited Listeria monocytogenes ATCC 19111 and Bacillus cereus ATCC 14579. Antimicrobial activity of culture supernatant was completely destroyed by proteinase K treatment, indicating the proteinaceous nature of the inhibitory substance. The
bacteriocin, Bac H27, was most stable at pH 7 and stable between pH 3-6 and 8-9 but inactive at pH 10 and above. The activity reduced to 50% after 15 min at 80A degrees C. Brain heart infusion MK-2206 in vitro (BHI) was the best medium for the Bac H27 activity (160 AU/mL). Bac H27 was purified by ammonium sulfate precipitation followed by Q-Sepharose and Sephadex G-75 column chromatographies. Its size was estimated to be 4.9 kDa by Tricine SDSPAGE. Partially purified Bac H27 killed L. monocytogenes ATCC 19111 when added into L. monocytogenes culture. Bac H27 could be useful as a novel food grade preservative inhibiting B. cereus and L. monocytogenes, 2 important food pathogens.”
“Damage occurring under tensile loading and the resulting increase in electrical resistance of tin-doped indium oxide and amorphous graphite coatings on various polymer substrates are investigated, using an electrofragmentation method
in situ in an optical microscope. The electrical resistance is modeled as a function of damage state assuming that the resistance of coating cracks is proportional to their opening, and Entinostat ic50 that there exists a conducting path of constant thickness at the coating/substrate interface across the crack. The model reproduces the experimental data with good accuracy apart from the damage initiation and saturation stages where it underestimates the measured coating resistance. This is due to the presence of stable cracks of finite length in the initiation stage and delamination in the saturation stage. Impedance spectroscopy measurements confirm the purely resistive nature of the conducting path, whose resistivity is found to be three to four orders of magnitude higher than that of the uncracked coating.