1 ± 15.42 years); group 2, exercise with VC supplementation (n = 28) (mean aged = 46.1 ± 11.35 years); group 3, exercise only (n = 26) (mean aged = 49.1 ± 15.9 years); and group 4, a control (n = 28) (mean aged = 49.9 ± 9.02 years). For all smokers, the baseline CBC results showed values within normal range (7.56 ± 1.78 x103 cc.mm

in WBC, 14.2 ± 2.34 g/dl in Hb, 45.68 ± 2.43% in Hct, 233.56 ± 58.32 × 105 in Plt, 55.31 ± 5.94% in Neutrophil, 32.50 ± 10.14% in lymphocyte, 2.56 ± 3.16% in monocyte, and 0.76 ± 0.62% in basophil respectively). Mean BMI of all groups were within Pevonedistat molecular weight the normal criteria of normal levels (18.5-24.9 kg m-2) according to the ACSM’Health-related physical fitness assessment manual find protocol [39]. Basic data showed that the smoking rate (cigarettes per day) was 5-10 (n = 21) and 11-20 (n = 9) in group 1,

5-10 (n = 13) and 11-20 (n = 15) in group 2, 5-10 (n = 12) and 11-20 (n = 14) in group 3, and 5-10 (n = 18) and 11-20 (n = 10) in group 4 (Table 1). Smoking rate (cigarettes per day) In this study, the cigarettes were divided into two types, light and self-rolled. In Figure 1, the yields of light and self-rolled cigarettes at the pre-intervention period were (5.93 ± 3.21 via 1.23 ± 2.01) in group 1, (8.68 ± 5.21 via 0.35 ± 2.34) in group 2, (7.46 ± 6.23 via 0.78 ± 1.11) in group 3, and (6.34 ± 2.20 via 0.98 ± 1.23) in group 4. After 2 months of intervention, results showed that the yield of cigarettes per day had reduced significantly to lower than that at the pre-intervention period in all groups, excepted group 4. The findings were 2.45 ± 4.67 (p < 0.05) via 0.56 ± 2.34 (p < 0.01) for group 1, 3.23 ± 4.32 (p < 0.01) via 0.21 ± 1.23 (p < 0.05) for group 2, 3.45 ± 2.21, (p < 0.01) via 0.45 ± 2.89, (p < 0.05) for group 3, and 7.23 ± 2.34 via 0.89 ± 1.34 for group 4 (p > 0.05). When calculating the percentage of cigarette reduction per day for both light and self-rolled types, it was reduced in all groups, excepted for group 4. Reduction values of (59.52%, and 54.47%) for group 1, (62.79%, and 40.00%) for group 2, (53.75%, and 42.30%)

for group 3. A 14.04% increase (light) 9.2% reduction (self-rolled) was noted for group 4. Figure 1 Cigarette yields per day of light (right) and self-rolling (left) types between pre- and post-intervention periods in each groups, control, VC, exercise with VC, and exercise. Each point represents Staurosporine in vitro the mean of cigarette yield per day. The percentage at post-intervention was compared to the pre-intervention. Oxidative Stress Biomarkers At the pre-intervention assessment, MDA and PrOOH were not difference between groups (Figure 2). The MDA levels of all groups had no significant difference, i.e. group 4 (2.34 ± 0.023 μmol/L), group 1 (2.45 ± 0.018 μmol/L), group 2 (2.32 ± 0.012 μmol/L), and group 3 (2.41 ± 0.023 μmol/L). After the two month intervention, the results showed a significant decrease in MDA for group 1 (1.89 ± 0.023 μmol/L, p < 0.