No patients received preoperative chemotherapy or radiotherapy, nor did any patients have histories of other treatments. Also, the tumors Afatinib EGFR were not associated with other inflammatory diseases. The pathology department in our hospital con firmed all 63 cases of surgically resected esophageal can cers and their adjacent normal esophageal tissues. The ages of the patients ranged from 45 79 years with a mean age of 73. 42 years. There were 19 cases younger than 60 years and 44 cases equal to or older than 60 years. The primary tumor was smaller than 3 cm in 36 cases and greater than or equal to 3 cm in 27 cases. In terms of differenti ation, 34 cases displayed high or intermediate differenti ation, whereas 29 cases displayed poor differentiation. There were 33 cases of pathological stage I II disease and 30 cases of stage III IV disease.

Thirty seven cases had lymph node metastasis, whereas 26 cases had no lymph node involvement. Distant metastasis was identified in 32 cases and was absent in 31 cases. Reagents To detect HtrA1 mRNA expression, the upstream pri mer P1 for Inhibitors,Modulators,Libraries HtrA1 was. The Inhibitors,Modulators,Libraries size of expected amplification product was 455 base pairs. According to the se quence of the human HtrA1 mRNA in GenBank and an analysis of its restriction digestion sites, we used the pri mer premier 5. 0 software to design a pair of primers to amplify the HtrA1 open reading frame. The up stream primer used for HtrA1 amplification was The expected amplification product was 451 bp. All of the above primers were synthesized by the Shanghai Invitro gen Biotechnology Company.

The primary antibody against HtrA1was a rabbit anti human polyclonal antibody, and the primary antibody against B actin was a mouse anti human monoclonal antibody. Both primary antibodies were purchased from Abcam, UK. The secondary antibodies Inhibitors,Modulators,Libraries were IRDye 800 conjugated, affinity purified, goat anti mouse IgG and IRDye 800 conjugated, affinity purified, goat anti rabbit IgG, both of which were purchased from the Odyssey Corporation. Inhibitors,Modulators,Libraries The vectors and the Trizol total RNA extraction kit were purchased from Invitro gen, USA. The restriction enzymes BamHI and XhoI and the DNA size marker were purchased from TaKaRa, Japan. The reverse transcription kit was purchased from Qiagen, Germany. The T4 DNA ligation kit was pur chased from Promega, USA. Taq DNA polymerase and pre stained protein molecular weight standards were purchased from Fermentas, USA.

Inhibitors,Modulators,Libraries The HtrA1 siRNA and the negative control siRNA were purchased from Sigma Aldrich, USA. The Eca 109 human esophageal cancer cell line was purchased from the Shanghai Institute of Cell Biol ogy, Chinese Academy of Sciences. RPMI 1640, trypsin, www.selleckchem.com/products/ldk378.html fetal bovine serum and the Lipofectamine 2000 transfec tion reagent were all purchased from Invitrogen, USA. Tissue culture plates and the Transwell invasion cham ber were purchased from the Corning Corporation.