Transplanting cells into the DoC, which subsequently form L drug activity against xenografts does not always correlate with its clinical activity. As seen in Figure 2, the parameters of drug dosage can be quickly and easily visual ized in the zebrafish. Furthermore, small molecular com pounds can be added directly to the environment of the zebrafish, which can be less stressful towards to the both the ani mal, and technician, compared to the injection techniques used in rodent models. One potential therapy using MMP inhibition was ana lysed in this study using the zebrafish model. MMPs have a critical role in inflammation and tumourigenesis Inhibitors,Modulators,Libraries and appear to be ideal as a drug target. Many inhibitors have been developed, and several have gone as far as clinical trials in cancer patients.

Unfortunately, though these inhibitors have showed Inhibitors,Modulators,Libraries promising effects in preclinical studies, the same agents did not have such a positive Inhibitors,Modulators,Libraries out a metastasis, has been shown to be controlled by the tumourigenic property of disseminated cells, and the microenvironment. However, it is important that the conditions are optimised for each cell type, including cell number, in order that the results are reliable and reproducible. This unique animal system provides a visual window into the metastastic process in a live vertebrate animal, with unprecedented clarity. The experiments described here establish the basis for the future Inhibitors,Modulators,Libraries development of a screening methodology for drugs that inhibit invasion and metastasis of breast cancers. Furthermore, the data also shows that transient transfection of siRNAs may be used to examine the effect of invasion and metastasis.

We have previously used the embryonic zebrafish xenograft model to study novel regulators Inhibitors,Modulators,Libraries of the TGF B signalling pathway, TNF receptor associated factor 4 and ubiquitin kinase inhibitor Volasertib specific protease 4, as well as the tumour suppressor FAF1, which in teracts with the FAS ligand. The zebrafish offers a promis ing future for functional studies of breast cancers. Studies of the efficacy of pharmacology and toxicology in murine xenograft models normally use tumour growth, body weight loss and mortality as parameters of toxicity. come in cancer treatment. This highlights the issue of complexity that the MMPs play in cancer progression. In this study, we used GM6001, a broad spectrum MMP inhibitor. GM6001 has been previously tested in the devel opment of zebrafish. These studies have shown the importance of MMPs during embryonic development and fin regeneration. We were able to show that MMP inhibition was capable of reducing the amount of in vasion and metastasis of human mammary carcinoma cells in the zebrafish. It must be noted that MMP inhibition was initiated at the early stage of cancer metastasis.