To measure the effect of age on accrued oxidative damage and Hsp70 expression we repeated the above experiments on foragers and nurses aged 30 to 32 days. We used mutual c-Met kinase inhibitor transfers of larvae between queen and worker driving conditions to find the proximate changes in expression of components of the insulin signaling pathway in response to changes in diet quality. Major changes were found by us as time passes and caste in 2 insulin like peptides and the insulin receptor. One insulin-like peptide was expressed at quite high levels in queen but not worker larvae. One other was expressed at higher levels in workers. The gene for an insulin receptor was expressed at higher levels in queen larvae than in worker larvae through the second larval instar, which precedes the identified differences in JH levels. These results demonstrate that the insulin pathway is compelling candidate for pursing the functional relationship between diet and the next hormonal signals involved in differentiation and caste determination. Influence of age and oxidative stress on expression in the honey-bee. to foraging for nectar and pollen beyond your colony. Nurses seldom travel, have Digestion relatively low metabolic rate, and experience homogeneous nest environment. In comparison, foragers create large thoracic temperatures during their frequent foraging trips and possess the highest measured mass specific metabolic rates. Therefore, foragers have six fold higher concentration of the strain protein Hsp70 within their thoraces than their heads, in addition to six and two fold fold higher Hsp70 levels than nurse thoraces and nurse heads. Apparently, temperature does not induce expression in forager thoraces at common trip conditions and sometimes even after exposure to 50C for 1h, temperature bees are unlikely to see in nature. In this ongoing study, we used the differences between nurse and forager honey bees to HSP70 inhibitor check the hypothesis that oxidative stress, as opposed to temperature stress, induces expression in forager thoraces. We measured carbonyl information, whole antioxidant activity, and expression of Hsp70 and several antioxidant enzymes, superoxide dismutase, catalase and glutathione s transferase, in thoraces and heads of 9 to11 day old foragers and nurses gathered as foraging activity begins, at middle day, or at end of the foraging day. We examined the aforementioned stress measures on thoraces and heads of foragers that have been collected just before making, or just after returning from foraging flight at each collection period, to look for the impact of simple foraging flight on tissue oxidative injury and Hsp70 expression.