Treatment of oral cancer cells with EGCG partly reversed the hypermethylation status of tumefaction suppressor gene RECK and improved the expression of RECKmRNA, which correlated with reduced expression of matrix metalloproteinases: MMP 2 and MMP 9 and suppressed the invasive capacity of cancer cells. Administration of black tea polyphenols notably paid down the incidence of DAB induced hepatomas in male Sprague Dawley rats, as shown by alterations in the expression of MMP 2, MMP 9, and TIMP 2, reversion inducing cysteine E2 conjugating abundant protein with Kazal motifs RECK, and reduction of HIF1alpha, VEGF, and VEGFR1 which correlated with HDAC1 levels. EGCG could inhibit DNMT action and reactivate methylation silenced retinoic p receptor B gene in human colon and prostate cancer cells. In yet another study,methylation of CDX2 and other genes concerned in gastric carcinogenesiswas investigated in relation to the clinico pathologic and selected life style facets of patients with gastric cancer. An inverse relationship of CDX2 methylation using the consumption of green tea was seen in this study. Reduced annexin I expression is a common event in early-stage bladder cancer development. Relatively, green tea extract caused the expression of protein and mRNA amounts of the actin binding protein, Endosymbiotic theory annexin I, through demethylation of its actin remodeling and ally. EGCG, an efficient inhibitor of human dihydrofolate reductase, improved the p16 methylation sample after folic acid deprivation leading to growth inhibition of a human colon carcinoma cell line in a concentration and timedependent fashion. The same research also demonstrated that through interruption of purine metabolism, EGCG caused adenosine release from the cells, and modulation of various signaling pathways via binding to adenosine specific receptors. EGCG induces apoptosis and inhibits growth in renal cell carcinoma through TFPI 2 mRNA and protein overexpression. PF299804 molecular weight Promoter demethylation of WIF 1 by epigallocatechin 3 gallate in lung cancer cellswas also described. Epigenetic silencing of glutathione S transferase pi by hypermethylation is known as being truly a feature of human prostate cancer. Recently, it has been reported that coverage of LNCaP cells to GTP concentrations as low as 1 10 ug/mL around 7 days caused demethylation in the proximal GSTP1 promoter and parts distal to the transcription factor binding web sites. This induced a concentration and timedependent re appearance of DNMT1 and GSTP1 inhibition. GTP exposure also elevated mRNA and protein levels of MeCP2, MBD4 and MBD1, and HDACs 1 3, while levels of acetylated histone H3 and H4 lowered.